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. 2017 Jul 18;8(39):64809–64826. doi: 10.18632/oncotarget.19363

Figure 5. Intermittent treatment cycles with 1 day of FTI followed by 3 days of SFN reverse nuclear shape alterations in HGPS cells.

Figure 5

A. Immunochemistry was performed on control (GMO3349C) and HGPS (HGADFN127) fibroblasts using antibodies directed against the indicated proteins (lamin B1 and progerin) after 2 cycles of 1 day of 0.06 µM FTI and 3 days of 1.0 µM SFN treatment or 3 cycles of 1 day of 0.06 µM FTI and 2 days of 1.0 µM SFN. Single-drug treatment of 0.06 µM FTI or 1.0 µM SFN for 9 days was performed in parallel. Cells were treated daily with fresh medium. Representative images are shown (n = 4). Scale-bar: 20 µm. B. The same cells as in (A) were used to determine the frequency of misshapen nuclei (dysmorphic) by direct counts of an average of 900 nuclei (n = 4).