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. 2017 Jul 18;8(39):64809–64826. doi: 10.18632/oncotarget.19363

Figure 6. Intermittent treatment cycles with 1 day of FTI followed by 3 days of SFN prevent prelamin A accumulation and donut-shaped nuclei formation.

Figure 6

A. Immunochemistry was performed on control (GMO3349C) and HGPS (HGADFN127) fibroblasts using an anti-prelamin A antibody. Cells were treated for 2 cycles of 1 day 0.06 µM FTI and 3 days 1.0 µM SFN treatment or 3 cycles of 1 day 0.06 µM FTI and 2 days 1.0 µM SFN treatment. Single-drug treatment of 0.06 µM FTI or 1.0 µM SFN for 9 days was performed in parallel. Representative images are shown (n = 4). Scale bar: 20 µm. B. The same cells and conditions as in (A) were used to analyze donut-shaped nuclei by direct counts of lamin A-stained nuclei. An average of 1,000 nuclei were counted. Left panel shows representative image of nuclei that were considered donut-shaped nuclei. Right panel shows the quantification of donut-shaped nuclei relative to mock-treated control. Data are presented as the percentage ± S.D. (*p-value ≤ 0.05; n = 3).