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. 2017 May 4;8(39):64840–64852. doi: 10.18632/oncotarget.17609

Figure 2. RASAL1 regulates RasGAP activity and proliferation in HSCs.

Figure 2

a., Ras activity was determined by comparing the levels of active GTP-bound Ras (Ras-GTP) that bound to recombinant Raf protein in control and RASAL1-expressing LX2 cells, with or without PDGF treatment for 1 h as a positive regulator of Ras activity. Equal amounts of recombinant Raf protein in pull-down assays were confirmed by analyzing GST-Raf protein levels in the immunoprecipitates. Representative results of five independent experiments are shown. b., The phosphorylation levels of Erk were lower in RASAL1-expressing cells compared with control LX2 cells after PDGF stimulation. A representative result from five independent experiments is shown. Similar results were obtained in Li-90 cells. c., The proliferation rate of RASAL1-expressing cells was significantly lower than that of control LX2 cells after PDGF stimulation. The relative cell number was determined by adjusting the values obtained before PDGF treatment to 1. Data are means ± SD of three independent experiments. *, p < 0.05 (t-test).