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. 2017 May 4;8(39):64878–64891. doi: 10.18632/oncotarget.17612

Figure 1. The effects of ZnO treatments on intestinal morphology, and the ralative mRNA expressions of intestinal antioxidant enzymes, tight junction proteins and inflammatory cytokines.

Figure 1

A. The jejunum in different groups were stained by HE. Histology images of jejunal morphology were collected. B. The relative mRNA expressions of Cu-Zn SOD, GPX1, ZO-1, and Occludin were detected. As to MT, its relative mRNA expression was also measured in jejunum C.. The relative mRNA expressions of IGF-1, CDK4 and Caspase3 in jejunum of weaning piglets were also measured D.. In addition, the IFN-γ, IL-1β, IL-6, TNF-α, NF-κB and TGF-β were detected by quantitative RT-PCR E.. β-actin was used as an internal standard for normalization. Values are means ± SEMs, n = 6. Different superscripts within each group mean significant difference (P < 0.05). Control, corn-soybean meal basal diet; Cu-Zn SOD, Cu-Zn superoxide dismutase; GPX1, glutathione peroxidase 1; MT, metallothionein; IGF-1, insulin-like growth factor 1; Nano-ZnO, corn-soybean meal basal diet supplemented with 600 mg Zn/kg as nano zinc oxide; ZnO, corn-soybean meal basal diet supplemented with 2000 mg Zn/kg from zinc oxide.