Table 4. Primers used for quantitative RT-PCR. 1,2.
Target | Primers sequences, 5’-3’ | Size (bp) | AT2 | |
---|---|---|---|---|
Forward | Reverse | |||
Cu-Zn SOD | CAGGTCCTCACTTCAATCC | CCAAACGACTTCCASCAT | 255 | 60 |
GPX1 | TGGGGAGATCCTGAATTG | GATAAACTTGGGGTCGGT | 183 | 56 |
MT | GTGAATCCGCGTTGCTCTCTGCT | CTGTGGGGCAGGAGCAGTTGG | 138 | 60 |
ZO-1 | GAGTTTGATAGTGGCGTT | GTGGGAGGATGCTGTTGT | 298 | 52 |
Occludin | ATCAACAAAGGCAACTCT | GCAGCAGCCATGTACTCT | 157 | 56 |
Caspase3 | TGTGTGCTTCTAAGCCATGG | AGTTCTGTGCCTCGGCAG | 158 | 58 |
IGF-1 | TGCTTCCGGAGCTGTGATCT | CCGACTTGGCAGGCTTGA | 67 | 58 |
NFκB | AGCTTGCCGTGTCTGCTGCT | CCGCCAAGGAGATGTTGTCG | 118 | 60 |
CDK4 | AGACCTGAAGCCAGAGAACATTC | AAGATACAGCCAACGCTCCAC | 195 | 60 |
IFN-γ | GAGCCAAATTGTCTCCTTCTAC | CGAAGTCATTCAGTTTCCCAG | 140 | 56 |
TGF-β | GGACCTTATCCTGAATGCCTT | TAGGTTACCACTGAGCCACAAT | 133 | 60 |
IL-1β | TGAAGTGCCGCACCCAAAACCT | CGGCTCCTCCTTTGCCACAATCA | 175 | 62 |
IL-6 | CCTGTCCACTGGGCACATAAC | CAAGAAACAACCTGGCTCTGAAAC | 253 | 63 |
TNF-α | CATCGCCGTCTCCTACCA | CCCAGATTCAGCAAAGTCCA | 199 | 56 |
β-actin | CTGGAACGGTGAAGGTGA | TTTGGAAAGGCAGGGACT | 170 | 60 |
1 Abbreviations: CDK4, cyclin-dependent kinase 4; Cu-Zn SOD, Cu-Zn superoxide dismutase; GPX1, glutathione peroxidase 1; IGF-1, insulin-like growth factor 1; MT, metallothionein; ZO-1, zonula occludens protein-1
2 AT: Annealing temperature in °C.