Table 4. Primers used for quantitative RT-PCR. ^1,2.

Target	Primers sequences, 5’-3’		Size (bp)	AT2
	Forward	Reverse
Cu-Zn SOD	CAGGTCCTCACTTCAATCC	CCAAACGACTTCCASCAT	255	60
GPX1	TGGGGAGATCCTGAATTG	GATAAACTTGGGGTCGGT	183	56
MT	GTGAATCCGCGTTGCTCTCTGCT	CTGTGGGGCAGGAGCAGTTGG	138	60
ZO-1	GAGTTTGATAGTGGCGTT	GTGGGAGGATGCTGTTGT	298	52
Occludin	ATCAACAAAGGCAACTCT	GCAGCAGCCATGTACTCT	157	56
Caspase3	TGTGTGCTTCTAAGCCATGG	AGTTCTGTGCCTCGGCAG	158	58
IGF-1	TGCTTCCGGAGCTGTGATCT	CCGACTTGGCAGGCTTGA	67	58
NFκB	AGCTTGCCGTGTCTGCTGCT	CCGCCAAGGAGATGTTGTCG	118	60
CDK4	AGACCTGAAGCCAGAGAACATTC	AAGATACAGCCAACGCTCCAC	195	60
IFN-γ	GAGCCAAATTGTCTCCTTCTAC	CGAAGTCATTCAGTTTCCCAG	140	56
TGF-β	GGACCTTATCCTGAATGCCTT	TAGGTTACCACTGAGCCACAAT	133	60
IL-1β	TGAAGTGCCGCACCCAAAACCT	CGGCTCCTCCTTTGCCACAATCA	175	62
IL-6	CCTGTCCACTGGGCACATAAC	CAAGAAACAACCTGGCTCTGAAAC	253	63
TNF-α	CATCGCCGTCTCCTACCA	CCCAGATTCAGCAAAGTCCA	199	56
β-actin	CTGGAACGGTGAAGGTGA	TTTGGAAAGGCAGGGACT	170	60

¹ Abbreviations: CDK4, cyclin-dependent kinase 4; Cu-Zn SOD, Cu-Zn superoxide dismutase; GPX1, glutathione peroxidase 1; IGF-1, insulin-like growth factor 1; MT, metallothionein; ZO-1, zonula occludens protein-1

² AT: Annealing temperature in °C.