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. 2017 May 10;8(39):65077–65089. doi: 10.18632/oncotarget.17779

Figure 3. SHP-1/p-STAT3 mediates SC-43-induced apoptosis in CCA cells.

Figure 3

SHP-1 plays a role in SC-43-induced STAT3 inhibition and apoptosis. (A) SC-43 increased SHP-1 activity in CCA cells. Cells were treated with SC-43 at the indicated doses for 16 hours. Columns, mean; bars, SD (n = 3). *P < 0.05; **P < 0.01. (B) SC-43 activates SHP-1 directly. SC-43 increases the phosphatase activity of SHP-1 in IP-SHP-1 cell lysate from HuCCT-1 cells. Columns, mean; bars, SD (n = 3). *P < 0.05; **P < 0.01. (C) Protective effects of SHP-1 specific inhibitor (PTPIII) on SC-43-induced apoptosis in HuCCT-1 and KKU-100 cells. Cells were pretreated with PTPIII at 100 nM for 30 minutes before SC-43 treatment. (D) left, knockdown of SHP-1 reduces the effects of SC-43 on p-STAT3 and apoptosis. HuCCT-1 cells were transfected with control siRNA or SHP-1 siRNA for 24 hours and then treated with SC-43 for another 24 hours. Right, overexpression of SHP-1 induces more apoptosis with SC-43. Apoptotic assay was performed by sub-G1 analysis. Columns, mean; bars, SD (n = 3). **P < 0.01.