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. 2017 May 9;8(39):65123–65131. doi: 10.18632/oncotarget.17729

Figure 1. PA variants with defects in functional pore formation.

Figure 1

(A) Schematic representation of the role of the interaction between residues Lys397 and Asp426 from adjacent PA protomers in PA functional pore formation. For simplicity, only octamers are shown. (B) Cytotoxicity of concentrated culture supernatants from PA variant-expressing BH480 strains. B16-BL6 cells cultured in 96-well plates were treated with various dilutions of the concentrated supernatants, or the indicated combinations of the supernatants from the PA variant-transformed BH480 strains, in the presence of 100 ng/ml FP59 for 48 h. MTT assay was used to evaluate cell viabilities relative to the non-toxin treated cells. Concentrated supernatant from a non-transformed BH480 strain was used as a control. Only the combination of PA-L1-D426K and PA-U2-K397Q demonstrated toxicity to the cells in the presence of FP59. (C) Schematic representation of the intermolecular complementation abilities of PA-L1-D426K and PA-U2-K397Q in PA functional pore formation. No functional pore is formed when PA-L1-D426K or PA-U2-K397Q is used alone. The functional pore shown is an idealized one in which the two PA variants alternate in the structure, whereas in reality they are expected to be randomly arranged.