Table 1. Binding kinetics of CD44v6-binding scFvs. Average association- and dissociation rate constants from the indicated number of replicate measurements are shown with standard deviations. Dissociation equilibrium constants were calculated as kd/ka.
| Candidate | ka (M-1s-1) | kd (s-1) | KD (nM) | Number of replicates |
|---|---|---|---|---|
| CD44v6-scFv-A7 | 2.1(±0.7)*105 | 5.7(±0.9)*10-3 | 27 | 4 |
| CD44v6-scFv-A8 | 0.9(±0.1)*105 | 3.3(±0.2)*10-3 | 37 | 4 |
| CD44v6-scFv-A11 | 2.1(±0.6)*105 | 1.7(±1.0)*10-4 | 0.8 | 8 |
| CD44v6-scFv-B4 | 3.7(±1.1)*105 | 3.8(±1.1)*10-4 | 1.0 | 6 |
| CD44v6-scFv-B5 | 0.5(±0.1)*105 | 3.5(±0.4)*10-3 | 70 | 2 |
| CD44v6-scFv-D1 | 5.1(±2.4)*105 | 9.9(±1.9)*10-4 | 1.9 | 6 |
| CD44v6-scFv-H12 | 5.5(±1.1)*105 | 2.7(±0.7)*10-4 | 0.5 | 6 |