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. 2017 May 25;8(39):65230–65239. doi: 10.18632/oncotarget.18191

Figure 1. Knockdown A20 reduced eNOS expression in TNFα -stimulated HUVECs.

Figure 1

HUVECs were treated by TNFα (20 ng/ml) for 0, 30, 60 and 120 mins with or without AdsiA20. (A and B) The mRNA and protein levels of phosphate-eNOS (p-eNOS) were detected by real-time PCR and Western blotting. (C and D) p-eNOS expression with pre-treatment of AdsiA20 or TNFα. (E and F) Relative quantification of nitric oxide production using nitrate reductase method in HUVECs pretreated with or without AdsiA20. (G) p-eNOS expression with pre-treatment of AdsiA20 or AdsiTAK1 in the absence of TNFα. (H) The percentage of apoptotic cells detected by TUNEL staining. *P < 0.05 versus TNFα-induced group at 0 h, respectively. Ad-sc: adenovirus-scramble siRNA;#P<0.05 versus TNFα-induced adenovirus-scramble siRNA group treated with 2 hours (n=6 independent experiments).