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. 2017 May 25;8(39):65230–65239. doi: 10.18632/oncotarget.18191

Figure 3. A20 was required for TNFα-caused TAK1/eNOS signalling in HUVECs.

Figure 3

(A) A20 or TAK1 expressions were examined in HUVECs pre-treated with AdsiA20 siRNA (it’s scramble siRNA as negative control), AdA20 or AdsiTAK1. (B and C) The expressions of TAK1 phosphorylation and p-eNOS were measured in HUVECs induced by TNFα, pre-treated by AdsiTAK1, or AdA20. (D and E) The effect of A20 on TNFα-induced TAK1 phosphorylation and p-eNOS expression after pre-treated with AdsiTAK1 or AdA20. (F) The effect of A20 on TNFα-induced HUVECs apoptosis after pre-treated with AdsiA20, AdA20 or AdsiTAK1. (G) Relative quantification of NO release using nitrate reductase method in HUVECs pre-treated by different conditions. *P<0.05 versus control Group, respectively (n=6 independent experiments).