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. 2017 May 17;8(39):65265–65280. doi: 10.18632/oncotarget.17941

Figure 9. The overexpression of Twist1 in esophageal normal fibroblasts enhanced in vivo tumorigenicity of esophageal cancer cells.

Figure 9

(A) TE11 cells were subcutaneously grafted to immune-compromised mice with tumor cells alone, or GFP-expressing esophageal normal fibroblast (ENF8), or Twist1-expressing ENF8. (B) Overexpression of Twist1 in esophageal normal fibroblast (ENF8) significantly enhanced the growth of TE11 (ESCC cells) in vivo. (C) Histologic examination revealed that TE11 xenograft tumor exhibited more infiltrative tumor growth when co-injected with Twist1-expressing esophageal fibroblast rather than ENF8-GFP or tumor cell alone (TE11 only). Scale bar indicates 100 μm. (D) Endolymphatic tumor emboli (left panel) and micropapillary pattern with tumor budding (right panel) were observed only in xenograft infused with both esophageal cancer cell (TE11) and Twist1-expressing esophageal fibroblasts (ENF8-Twist1) Scale bar indicates 100 μm.