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. 2017 Jun 27;8(39):65339–65358. doi: 10.18632/oncotarget.18689

Figure 5. Transport of DIM nanoformulation through in vitro and in vivo BBB.

Figure 5

(A) Pictorial representation of in vitro BBB model. (B) ICAM1 staining of isolated primary rat brain endothelial cells. (C) Fluorescence imaging of C6 astrocytes shows binding of FITC-tagged SSTR2 pep-DIM-NPs passed through the tight junction of in vitro BBB. Bright field (BF) image shows C6 cells on the lower side of transwell insert. (D) Fluorescence (Upper), inverted (middle) and bright field (lower) imaging of multiple organs [brain (i), heart (ii), liver (iii), kidney (iv), Pancreas (v), spleen (vi) and lung (vii)] of control and tumor bearing rats represent binding of the TAMRA tagged-SSTR2 pep-DIM-NPs to the specific brain tumor region. (E) Enlarged images of rat brains shows enhanced and specific binding of nanoformulation to the tumor region (denoted by black arrow), which is absent in control rat brain (lower panel).