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. 2017 Jun 16;8(39):65397–65406. doi: 10.18632/oncotarget.18541

Figure 1. Generation of miR-149* mutant mice by using the CRISPR/Cas9 system.

Figure 1

(A) Schematic of the mouse miR-149* sequence (mmu-miR-149*) and the binding sites of the sgRNA. (B) Detection of mutations in F0 mice by T7E1 digestion using PCR products amplified from ear genomic DNA. Cleavage bands indicate the presence of mutations in the miR-149* gene in F0 mice. (C) The DNA sequence of the mutant miR-149* gene in founder #6 of F1 mice. Four TA clones of the PCR products amplified from each founder were analyzed by DNA sequencing. sgRNA sequences are underlined. The short dash lines indicate deletion of nucleotides.