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. 2017 Jul 1;8(39):65445–65456. doi: 10.18632/oncotarget.18920

Figure 2. PHB1 and PHB2 co-localize to mitochondria of Kit225 cells.

Figure 2

Kit225 cells were cytocentrifuged onto glass slides and subjected to analysis by immunofluorescent confocal microscopy. (A) PHB subcellular localization was determined by staining the nucleus with DAPI (panel 1); PHB1-Cy2 (panel 2) and PHB2-Cy3 (panel 3) and overlay (panel 4). (B) Nuclear staining with DAPI (panel 1), OxPhos CII-Cy2 (panel 2), PHB2-Cy3 (panel 3) and overlay (panel 4) were detected in Kit225 cells using immunofluorescent confocal microscopy.