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. 2017 Aug 7;8(39):65627–65641. doi: 10.18632/oncotarget.20019

Figure 6. The cell proliferation assay after Mt2 overexpression in GC-1 spg cells.

Figure 6

Overexpression plasmid of Mt2 was constructed using pCMV6 plasmid. Plasmid was transfected into GC-1 spg cells with Lipofectamine 2000. Both mRNA (A) and protein (B) of Mt2 were increased in cells with Mt2 overexpression. Furthermore, cell proliferation assay showed that Mt2 overexpression promoted cell proliferation and the expression of genes associated with cell proliferation (C, D). ★ indicates significant differences (p < 0.05). ★★ indicates extremely significant differences (p < 0.01).