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. 2017 Aug 7;8(39):65687–65698. doi: 10.18632/oncotarget.20058

Figure 7. MiR-324-3p enhances activation of Wnt/β-catenin signaling pathway via targeting DACT1 in HCC cells.

Figure 7

Western blot results and according statistical graphs revealed that both cytoplasmic and nuclear β-catenin expressions were increased in SMMC-7721 cells with miR-324-3p overexpression, while decreased in Hep3B cells with miR-324-3p knockdown. Both cytoplasmic and nuclear β-catenin expressions could be reversed by DACT1 clone or siRNA compared to the control groups. Meanwhile, the expression changes of c-Myc and cyclin D1, downstream targets of Wnt/β-catenin pathway, could be positively regulated by miR-324-3p in HCC cells. These regulatory effects could be reversed by DACT1. n = three repeats with similar results, *P < 0.05, **P < 0.01, ***P < 0.001 by ANOVA.