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. 2017 Aug 9;8(39):65699–65716. doi: 10.18632/oncotarget.20081

Table 4. Primer sequences of genes used for real time quantitative PCR.

Symbol Gene description Forward primer Reverse primer
GAPDH glyceraldehyde-3-phosphate dehydrogenase TGCCCTCAACGACCACTTT GGTCCAGGGGTCTTACTCCTT
DNMT1 DNA methyltransferase 1 CCTGTACCGAGTTGGTGATGGT CCTTCCGTGGGCGTTTC
DNMT3A DNA methyltransferase 3 alpha CTCCTGTGGGAGCCTCAATGTTACC CAGTTCTTGCAGTTTTGGCACATTCC
DNMT3B DNA methyltransferase 3 beta ACCACCTGCTGAATTACTCACGC GATGGCATCAATCATCACTGGATT
MBD2 methyl-CpG binding domain protein 2 CCATGGAACTACCCAAAGGTCTT CAGCAGATAAAAGGGTCTCATCATT
MBD4 methyl-CpG binding domain 4, DNA glycosylase TCTAGTGAGCGCCTAGTCCCAG TTCCAATTCCATAGCAACATCTTCT
TET1 tet methylcytosine dioxygenase 1 AATGGAAGCACTGTGGTTTG ACATGGAGCTGCTCATCTTG
TET2 tet methylcytosine dioxygenase 2 AATGGCAGCACATTGGTATG AGCTTCCACACTCCCAAACT
TET3 tet methylcytosine dioxygenase 3 TTGCGTCGAACAAATAGTGG CCCGTGTAGATGACCTTCTC
SAT1 spermidine/spermine N1-acetyltransferase 1 GGTTGCAGAAGTGCCGAAAG GTAACTTGCCAATCCACGGG