| Construction of mutants |
|
calR-A |
GTAGCTGCAGGCAGATTATTTGACTGATACGC |
|
calR-B |
GTTCGCAAATGGGAAGTCTCTCATCGCATCTTTCTTCTC |
|
calR-C |
GAGAAGAAAGATGCGATGAGAGACTTCCCATTTGCGAAC |
|
calR-D |
GTGAGCATGCTACTTACCTTTTGGCTTACAG |
|
toxR-A |
GTGACTGCAGAAACGCAATTTGTCTGATG |
|
toxR-B |
ATCTTCATGCTGGCCTCCTTTAGTTCTTCTTAGATGGATGATG |
|
toxR-C |
CATCATCCATCTAAGAAGAACTAAAGGAGGCCAGCATGAAGAT |
|
toxR-D |
GTGAGCATGCAATTCGGCGGCTTTGTTC |
| Construction of complementary strain |
|
calR-HP-F |
GCGGTCGACAGGAGGAATTCACCATGTTAGAGAAGAAAGATG |
|
calR-HP-R |
GCGAAGCTTTTATTTTGATGCGACCAC |
|
toxR-HP-F |
GATTCTAGAAGGAGGAATTCACCATGACTAACATCGGCACCAA |
|
toxR-HP-R |
GACAAGCTTTTATTTGCAGATGTCTGTTGG |
| Protein expression |
|
calR-P-F |
GCGGGATCCATGTTAGAGAAGAAAGATG |
|
calR-P-R |
GCGAAGCTTTTATTTTGATGCGACCAC |
|
toxR-P-F |
AGCGGGATCCATGACTAACATCGGCACCAA |
|
toxR-P-R |
AGCGAAGCTTTTAAGGATTCACAGCAGAAG |
| qRT-PCR |
|
calR-RT-F |
ATGTAAAAAGAAAACCGTACA |
|
calR-RT-R |
AACACAGCAGAATGACCGTG |
|
toxR-RT-F |
TTGTTTGGCGTGAGCAAGG |
|
toxR-RT-R |
TAGCAGAGGCGTCATTGTTATC |
|
exsB-RT-F |
ATGAAAAGCAGTAAGTGGGC |
|
exsB-RT-R |
CTGAGAAGCAACAGTAAGAC |
|
vp1687-RT-F |
TGCTCACCGTTGCCAAATAG |
|
vp1687-RT-R |
GCGACGCTTTCATGTATTGC |
|
vp1667-RT-R |
GGAATGGATTGGAATCGTC |
|
vp1667-RT-R |
CCACCGTCTTTTATTTTGC |
| 16S rDNA-RT-F |
GACACGGTCCAGACTCCTAC |
| 16S rDNA-RT-R |
GGTGCTTCTTCTGTCGCTAAC |
| Primer extension |
|
calR-PE-R |
GCAAAATATCGGTACTTCA |
|
toxR-PE-R |
TTAGTTCTTCTTAGATGGATGATG |
|
exsB-PE-R |
GTCTTATTATGATTTATTTTTACAC |
|
vp1687-PE-R |
GGCAACGGTGAGCAAAATC |
|
vp1667-PE-R |
GACGATTCCAATCCATTCCG |
| LacZ fusion |
|
calR-lacZ-F |
GCGGTCGACGTTTGTTTGCTCGGATTGTTTG |
|
calR-lacZ-R |
GCGTCTAGACAAAGTGCTTTCCATACGGTAG |
|
toxR-lacZ-F |
GCGCGTCGACATCGTTAAGGTATTTGCA |
|
toxR-lacZ-R |
GCGCGAATTCCGAGCGAATTACTATTTGG |
|
exsB-lacZ-F |
ATATGTCGACATTGTCCGTCAAATGCAGTTC |
|
exsB-lacZ-R |
TTTTGAATTC CATATACATTCGCTTGGCTCTG |
|
vp1687-lacZ-F |
GCGCGTCGACGCATTATTGACGCCAGTATCG |
|
vp1687-lacZ-R |
GCGCTCTAGAGGCAACGGTGAGCAAAATC |
|
vp1667-lacZ-F |
GCGGTCGACCAGATTGCTGAATATCGGTG |
|
vp1667-lacZ-R |
GCGTCTAGA AAGCGATTGAGTGGCGTTG |
| EMSA |
|
calR-EMSA-F1 |
GTTTGTTTGCTCGGATTGTTTG |
|
calR-EMSA-R |
CAAAGTGCTTTCCATACGGTAG |
|
toxR-EMSA-F |
ATCGTTAAGGTATTTGCA |
|
toxR-EMSA-R |
CGAGCGAATTACTATTTGG |
|
exsB-EMSA-F |
ATTGTCCGTCAAATGCAGTTC |
|
exsB-EMSA-R |
CATATACATTCGCTTGGCTCTG |
|
vp1687-EMSA-F |
GCATTATTGACGCCAGTATCG |
|
vp1687-EMSA-R |
GGCAACGGTGAGCAAAATC |
|
vp1667-EMSA-F |
CAGATTGCTGAATATCGGTG |
|
vp1667-EMSA-R |
AAGCGATTGAGTGGCGTTG |
| DNase I footprinting |
|
calR-FP-F |
CAGATTGCTGAATATCGGTG |
|
calR-FP-R |
ATTGATAATACTCATTCACTTGC |
|
calR-FP-F (M13F) |
GTAAAACGACGGCCAGTCCGTTGGTTATTGATAG |
|
calR-FP-R (M13R) |
CAGGAAACAGCTATGACCCACGGCATTACTTACTG |
|
toxR-FP-F (M13F) |
GTAAAACGACGGCCAGTTTTCAGGGACGACTTTGTG |
|
toxR-FP-R (M13R) |
CAGGAAACAGCTATGACCGAGCGAATTACTATTTGG |
|
exsB-FP-F (M13F) |
GTAAAACGACGGCCAGTGTTTATCAATTTTGGTTGTTAG |
|
exsB-FP-R (M13R) |
CAGGAAACAGCTATGACCGGCTTATATTTATTCTAC |
|
vp1687-FP-F (M13F) |
GTAAAACGACGGCCAGTCACCAGAGTAGGGCATCAC |
|
vp1687-FP-R (M13R) |
CAGGAAACAGCTATGACCAAGCCAATGAGCGTCAG |
|
vp1667-FP-F (M13F) |
GTAAAACGACGGCCAGTCAGATTGCTGAATATCGGTG |
|
vp1667-FP-R (M13R) |
CAGGAAACAGCTATGACAAGCGATTGAGTGGCGTTG |
| M13F-FAM |
GTAAAACGACGGCCAGT |
| M13R-HEX |
CAGGAAACAGCTATGAC |
