Table 1. The effect of DSF:Cu and ionising radiation on cellular accumulation at the G2 checkpoint.
Control | DSF:Cu | 5 Gy | DSF:Cu + 5 Gy | |
---|---|---|---|---|
SK-N-BE(2c) | 30.5 ± 0.63 | 34.5 ± 1.63 | 53.7 ± 5.05* | 40.4 ± 0.51¶ |
UVW | 23.0 ± 0.83 | 33.9 ± 2.57** | 56.1 ± 1.40*** | 44.0 ± 0.50††¶¶ |
The percentages of unsynchronised SK-N-BE(2c) and UVW cells in G2-M, 12 h after irradiation and exposure to 0.3 μM DSF:Cu, were determined by FACS analysis of propidium-iodide-stained cells. Data are means ± SEM, n=3. One-way ANOVA with Bonferroni correction was used to compare the means of the test groups with those of the untreated control (*), with those of the irradiated group (†) or compared with those treated with DSF:Cu alone (¶). One symbol: P<0.05; two symbols: P<0.01; three symbols: P<0.001.