Figure 5. In vitro characteristics of ⁸⁹Zr-DFO-MPA1.

(A) Representative radio-ITLC (instant thin layer chromatography) of ⁸⁹Zr-DFO-MPA1 showing high radiochemical purity (>99%) as ⁸⁹Zr-DFO-MPA1 remains at baseline and free ⁸⁹Zr was undetectable (developed in 50mM DTPA). (B) Competitive binding assay of ⁸⁹Zr-DFO-MPA1 in THP-1 cells indicate no significant loss of affinity during radiolabeling. Radiotracer binding gradually decreases with incremental increase of unlabeled MPA1 blocking (IC₅₀=11.51 nM).