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. 2017 Jul 26;8(39):65932–65945. doi: 10.18632/oncotarget.19582

Figure 3. Effect of miR-132 on Shh expression.

Figure 3

(A) The sequences of wild-type Shh and the mutant that disrupts the interaction with miR-132 are shown. The histogram bars represent the relative dual luciferase activity in HCC cells transfected with miR-132 mimics or miR NC (n = 3, *p < 0.05 for miR-132 mimics vs. miR NC). (B) The relative mRNA levels of Shh were determined by RT-PCR (n = 3, *p < 0.05 for miR-132 mimics vs. miR mimics control). The protein expression of Shh was measured by western blotting. (C) The gene and protein expression were detected by qRT-PCR or western blotting in LM3 and HepG2 cells transfected with Shh siRNAs (n = 3, **p < 0.01 for si-Shh #2 vs. si-Shh NC). (D) Apoptosis of LM3 and HepG2 cells was determined by flow cytometry. (E) The protein expression of cleaved caspases was measured by western blotting.