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. 2017 Jul 26;8(39):65932–65945. doi: 10.18632/oncotarget.19582

Figure 4. TUG1 affects Shh protein expression by targeting miR-132.

Figure 4

(A) The relative mRNA expression of TUG1 and miR-132 in HCC cells and tissues was detected by qRT-PCR (n = 3, *P 0.05 and **P < 0.01 for HCC cells versus LO2). Statistical correlations between the expression of miR-132 and TUG1 were analyzed by Spearman’s test in SPSS 20.0 software (R2 = 0.706 for cells and R2 = 0.828 for tissues). (B) LM3 and HepG2 cells were transfected with TUG1 siRNAs (100 nm). The CCK8 kit was used to monitor cell proliferation (n = 3). The protein expression of Shh was measured by western blotting. (C) The expression of TUG1, miR-132, Shh, and pri-miR-132 was detected by qRT-PCR and western blotting in cells transfected with TUG1 siRNAs (n = 3, **p < 0.01 for si-TUG1 versus si-TUG1 NC). (D) Sequences of wild-type TUG1 and the mutant that disrupts the interaction with miR-132 are shown. The histogram bars represent the relative dual luciferase activity in HCC cells transfected with miR-132 mimics or miR NC (n = 3, *p < 0.05 for miR-132 mimics versus miR NC).