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. 2017 Jul 27;8(39):65969–65982. doi: 10.18632/oncotarget.19622

Figure 3. EGF upregulates the expression of MMP-9 and the activities of ERK1/2, AKT, STAT3, and STAT5 pathways via EGFR.

Figure 3

(A, B) A172 cells were treated with EGF (10 ng/ml) in the absence or presence of EGFR siRNA for 48 h. Total mRNA, total protein, and conditioned medium were collected. (A) Expression levels of mRNA and protein and the activity of MMP-9 were determined by qRT-PCR, Western blotting, and zymography, respectively. GAPDH and MMP-11 served as internal controls. (B) Phosphorylation of ERK1/2, AKT, STAT3, and STAT5 were determined by Western blotting. Data represent the mean ± SE of at least 3 independent experiments. *, P < 0.05 versus vehicle-treated controls; #, P < 0.05 versus cells treated with EGF alone.