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. 2017 Aug 12;8(39):66328–66342. doi: 10.18632/oncotarget.20240

Figure 2. Detection of NKCC1 in MHCC97 cell lines and HCC tissues.

Figure 2

(A) Western blotting results validated the upregulation of NKCC1 in highly metastatic HCC cell lines. The triangle indicates the sequentially increased metastatic abilities of the MHCC97L, MHCC97H and HCCLM6 cell lines. * p<0.05 or ** p<0.01 indicates a significant difference of NKCC1 expression level between HCC cell lines (one-way ANOVA). (B) The representative immunohistochemistry staining results of NKCC1 in HCC tumor tissue. (F-a) Result of sample W2 as the representative staining of (-). Weak and partial plasma membrane staining in ≤10% cancer cells. (F-b) Result of sample C7 as the representative staining of (1+). Weak and partial plasma membrane staining in >10% cancer cells. (F-c) Result of sample C1 as the representative staining of (2+). Moderate and partial plasma membrane staining in >10% cancer cells, or strong and complete plasma membrane staining in ≤10% cancer cells. (F-d) Result of sample C9 as the representative staining of (3+). Strong and complete plasma membrane staining in >10% cancer cells.