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. 2017 Aug 12;8(39):66328–66342. doi: 10.18632/oncotarget.20240

Figure 5. Expression levels of WNK1, OSR1, SPAK and NKCC1 in MHCC97L and MHCC97H cells.

Figure 5

The total and phosphorylated protein levels of WNK1, OSR1, SPAK, and NKCC1 were detected by Western blotting in HCC cell lines with sequentially increased metastatic abilities (MHCC97L<MHCC97H). Total protein levels (t-) and active phosphorylated protein levels (p-) of WNK1, OSR1, and NKCC1 were all significantly increased in MHCC97H. The total protein level of SPAK was significantly increased in MHCC97H, but the active phosphorylated protein level of SPAK remained unchanged. * p<0.05, ** p<0.01 or *** p<0.001 indicates a significant difference (two-tailed Student's t-test).