Figure 3.

BD750 inhibits proinflammatory cytokine secretion and STAT5 phosphorylation in DCs. BMDCs were stimulated with cytokines for expansion in the presence or absence of BD750 for 12 h and then stimulated with LPS for 24 h. The supernatants of cultured cells were harvested and the levels of IL-6, IL-10, IL-12, TNF-α, IL-1β and IL-23 in the supernatants were determined by ELISA (A–F). In addition, the relative levels of STAT5 and AKT expression and phosphorylation in the different groups of DCs were determined by Western blot assays (G–H). The relative levels of STAT5 and AKT phosphorylation to total STAT5 and AKT were determined. The relative density for bands of p-STAT5 or p-AKT activity was normalized to the total STAT5 or AKT, respectively. Data are representative images or expressed as the mean – SEM of three independent experiments. *P < 0.05, **P < 0.01 versus the mDC group.