Figure 4.

BD750-treated DC impairs allogeneic antigen-stimulated T cell proliferation in vitro. ImDCs, mDCs and BD750-pretreated DCs (12 h) were mixed in triplicate with carboxyfluo-resceinsuccinimidyl ester–labeled allogeneic naive CD4⁺CD25^– T cells for 72 h at different ratios (DC:CD4 + T, 1:10, 1:30 and 1:100), and the proliferation of T cells was determined by flow cytometry. Data are representative histograms or expressed as the mean ± SEM of three independent experiments. (A) Flow cytometry analysis. (B–D) Quantitative analysis. *P < 0.05, **P < 0.01 versus the mDC group.