Figure 5.

BD750-treated DC impairs allogeneic antigen-stimulated Th1 and Th17 responses in vitro. Following MLR in vitro, the frequency of Th1 and Th17 cells and Tregs was determined by flow cytometry. The levels of IL-17A and IFN-γ in the supernatants of cocultured T and DC cells were determined by ELISA. Data are representative FACS charts or expressed as the mean ± SEM of three independent experiments. (A) Flow cytometry analysis of Th1 and Th17 cells and Tregs. (B–D) Quantitative analysis of Th1 and Th17 cells and Tregs. (E–G) ELISA analysis of the levels of IL-17A, IFN-γ and IL-10. *P < 0.05, **P < 0.01 versus the mDC group.