Figure 7.

Adoptive transfer of BD750-treated DC decreases the proportion of splenic Th1 and Th17 cells and Tregs in EAE mice. On d 22 post the first PTX injection, some mice from each group were euthanized and their splenic T cells were stained with anti-CD4/anti-CD25, intracellularly stained with anti-IL-17A, anti-IFN-γ or anti-Foxp3. The percentages of splenic Th1 and Th17 cells and Tregs were determined by flow cytometry. Data are representative FACS charts or expressed as the mean ± SEM of each group of mice (n = 8 per group). (A) Flow cytometry analysis. (B) Quantitative analysis of the percentages of Th1 and Th17 cells and Tregs. *P < 0.05, **P < 0.01 versus the PBS group.