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Bio-Plex analysis and experimental design. Secretome analysis was performed on supernatants collected at d 1 (no cytokines), d 14 and d 21 of ex vivo expansion of CIK cells. The right panel shows the complete list of growth factors, chemokines and cytokines analyzed by Bio-Plex. IFN-γ (1000 U/mL) was added at d 0, while OKT3 antibody (50 ng/mL) and IL-2 (300 U/mL) were added at d 1 and up to the end, refreshing the medium every 2–3 d.
