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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: J Neurochem. 2017 Aug 4;143(2):236–243. doi: 10.1111/jnc.14110

Figure 2. Granulin D and E bind to PSAP with similar affinity as full length PGRN.

Figure 2

(A) Conditioned medium containing AP, AP-PGRN, AP-Grn D, or AP-Grn E were incubated with COS-7 cells transfected with PSAP fused to PDGFR. Cells were fixed and AP binding was visualized with AP substrates. Scale bar=100μm. (B) AP–PGRN binding to PSAP-PDGFR expressing COS-7 cells measured as a function of AP–PGRN concentration. (C-E) Scatchard plot of AP-PGRN (C), AP-GRN D (D) or AP-GRN E (E) binding to PSAP-PDGFR expressing COS-7 cells. KD, mean ± sem, n = 4.