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. 2017 Oct 6;7:12775. doi: 10.1038/s41598-017-13054-3

Figure 1.

Figure 1

Ramp currents generated by TRPV1 are sensitized by pBQ and NAPQI. Traces show voltage ramp-induced membrane currents (see insert) in hTRPV1-expressing HEK 293 cells, one representative recording out of 6–7 measured cells of each group. (A) Sensitization of hTRPV1 by 1 µM pBQ is dependent on the duration of application, a maximum is reached at about 300 s. 1 µM capsaicin was used as control. (B) 100 nM BCTC blocks pBQ (5 min)-induced currents. Note that currents slowly recover following washout of BCTC for 2 minutes. (C) 1 µM NAPQI (5 min) sensitizes ramp currents generated by TRPV1, and these can be transiently blocked by BCTC. (D) Increasing concentrations of acetaminophen (APAP 10 µM, 100 µM, 1000 µM, each applied for 4 minutes) has no effects on ramp currents evoked in hTRPV1-expressing cells.