Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 6;7:12781. doi: 10.1038/s41598-017-12989-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The effect of read length and sequencing depth on the technical error variability using simulated scRNA-seq datasets. A: Number of identified expressed genes (Fragment per Kilobase per Million reads; FPKM >1) as a function of read length and sequencing depth (A). Error bars (box plot, mean and 5–95% interval) represent variability across individual cells. (B) Mean pairwise cell-to-cell Pearson correlation of gene expression values as a function of sequencing depth and read length. (C) The distribution of pairwise cell-to-cell Pearson correlation of gene expression values using subsets of different read length drawn from the original dataset. Original dataset had a read length of 145 bp with depth >8 millions PE reads, two samples drawn from this dataset were taken, with length 25 bp and same depth.