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. 2017 Oct 6;7:12739. doi: 10.1038/s41598-017-10418-7

Figure 1.

Figure 1

Schematic outline of chromosome engineering technique, including transgene insertion and telomere-associated truncation of a human chromosome 21 in CHO-K1 cells and the transgene insertion of a human chromosome 8 in mouse A9 cells using CRISPR/Cas9. (a) Outline of a current chromosome engineering technique using DT40 cells. This technique requires three rounds of MMCT to transfer a human chromosome to DT40 cells and re-transfer the modified chromosome to CHO cells. The modified chromosome can then be transferred to recipient cells. (b) Outline of the improved technique using CRISPR/Cas9 requires one round of MMCT to directly transfer the modified chromosome to recipient cells.