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. 2017 Oct 6;7:12739. doi: 10.1038/s41598-017-10418-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative PCR and FISH results of HT1080 and B16F10 cells transferred with the modified hChr.21. (a) Representative results of transgene insertion analysed by PCR. (b) Representative FISH images of HT1080 Bsd #06-02 transferred with the targeted chromosome. The rhodamine (red) signal indicates the repetitive sequence of plasmid p11-4 containing human alpha-satellite sequences, which stains the centromeric region of human Chr. 21. The FITC signal (green) indicates the presence of the inserted I-EGFP-I-Bsd-Fcy; Fur vector on the near centromeric region. (c) Representative results of telomere-associated truncation analysed by PCR. (d) Representative FISH images of B16F10 HisD44-01. The rhodamine (red) signal indicates the repetitive sequence of human Cot-1 for staining of whole human chromosome. The FITC signal (green) indicates the presence of pBS-TEL/Dq-HisD vector on the same truncated hChr.21 as observed in the CHO donor cells. Full-length gels of the PCR analysis are presented in Supplementary Figure 2.