Table 2.
Summary of FISH analysis of the targeted chromosome transferred to HT1080 cells.
| Ploidy | 2n | |||
|---|---|---|---|---|
| Number of maintained hChr. 21 | 0 | 1 | 2 | |
| Clone # | HT1080 Bsd #06-01 | 12 | 25 | 2 |
| HT1080 Bsd #06-02 | 8 | 40 | 0 | |
| HT1080 Bsd #22-01 | 10 | 36 | 0 | |
| HT1080 Bsd #22-02 | 12 | 30 | 1 | |
| HT1080 Bsd #30-02 | 4 | 26 | 17 | |
| HT1080 Bsd #32-03 | 6 | 34 | 0 | |
| HT1080 Bsd #36-01 | 40 | 0 | 0 | |
Approximately 40 cells were observed to investigate whether the transferred chromosome contained the targeted vector and was maintained independently in HT1080 cells. Each column shows the number of such cells. The ploidy of the HT1080 host chromosomes was 2 n. The numbers of maintained hChr. 21 are shown in the second row.