Table 3.
Summary of PCR results for telomere-associated truncation.
| Clone # of CHO donor clone (positive/analyzed) | HisD #04 | HisD #21 | HisD #36 | HisD #44 |
|---|---|---|---|---|
| Primer pair #3 | 21/22 | 3/12 | 2/3 | 8/8 |
| Primer pair #4 | 0/22 | 12/12 | 2/3 | 0/8 |
| Primer pair #5 | 0/22 | 12/12 | 0/3 | 0/8 |
| Primer pair #6 | 0/22 | 10/12 | 0/3 | 0/8 |
| Correct clone | 21/22 | 0/12 | 1/3 | 8/8 |
| (%) | 95% | 0% | 33% | 100% |
Each column shows the number of the PCR-positive B16F10 clones for each primer pair out of the number of analysed clones. B16F10 clones derived from CHO HisD#4 showed that 21 of 22 were positive with primer pair #3; in contrast, all of the clones were negative with primer pairs #4, 5 and 6. Therefore, 21 of 22 analysed clones (95%) showed an ideal result for PCR, indicating successful truncation of hChr. 21.