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. 2017 Oct 6;7:12743. doi: 10.1038/s41598-017-12972-6

Figure 2.

Figure 2

Development of elevated spontaneous activity in S1 of the tSCI mice. (A).Representative traces of spontaneous AP firing in layer V pyramidal neurons in cortical slices from the HL-S1 of the sham group and at different times post-tSCI. Recordings were made at −60 mV in a modified ACSF that slightly enhanced spontaneous firing (n = 4–8 mice in each group). (B) Spike frequencies in different groups (1.72 ± 0.44 Hz, 0.26 ± 0.10 Hz, 1.23 ± 0.77 Hz, 1.72 ± 0.52 Hz, 3.22 ± 0.63 Hz, and 5.13 ± 0.77 Hz for the sham, 6 hour, 1 day, 3 day, 7 day and 14 day groups respectively). There was a significant decrease in spike frequency at 6 hours post-tSCI (p < 0.05 when compared with the sham group), which was followed by a progressive increase in spike frequency between 3–14 days post-tSCI. Spike frequency in the 3 day group was higher than 6 hour and 1 day groups, and spike frequencies in 7 day and 14 day groups were higher than the sham, 6 hour, and 1 day groups. *p < 0.05; **p < 0.01; *** < 0.001; Mann-Whitney test). The numbers on top are numbers of recorded neurons in each group.