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. 2017 Oct 6;8:797. doi: 10.1038/s41467-017-00842-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Impact of miR-221/222 processing on PTEN protein expression. a PTEN mRNA levels evaluated by qRT-PCR analysis of HeLa cell clones silenced for APE1 expression. Total RNA was extracted from HeLa cell clones expressing APE1 WT or APE1 silenced (siRNA) and reverse transcribed. Histogram shows the detected levels of PTEN normalized to GAPDH levels. Asterisks represent a significant difference with respect to control (SCR).*P < 0.05, Student’s t-test. b PTEN mRNA levels evaluated by qRT-PCR analysis of HeLa cell treated with 20 µM compound #3 or 100 µM E3330 for 24 h, respectively. Histogram shows the detected levels of PTEN normalized to GAPDH levels. Asterisks represent a significant difference with respect to control (NT). **P < 0.001, Student’s t-test. c PTEN protein level evaluated in HeLa cell clone silenced for APE1 expression. Representative western blotting analyses of total cell extracts of HeLa cell clones. PTEN expression inversely correlates with phosphorylation of Akt1 (pAkt1). Histogram reports expression level of PTEN and pAkt protein obtained after quantification of the signal intensity of the corresponding bands. Data represent the means of ± SD of three independent experiments. Tubulin was used as loading control and for data normalization. Asterisks represent a significant difference with respect to control (SCR).*P < 0.05, **P < 0.001, Student’s t-test