Fig. 2.

Reduced diffusion of glycosylated human IFNγ and IL-12 through galectin-3-loaded collagen matrices. a Schematic drawing of the protocol. Galectin-3 (1 μg per transwell), IFNγ (25 ng per transwell), IL-12 (7 ng per transwell), and LacNAc (1.2 mg per transwell). b IFNγ diffusion through collagen in 4 h was measured by ELISA. Mean ± SD of three experiments performed in duplicates. P = 0.006; Kruskal–Wallis test with Dunn’s Multiple Comparison Correction Test. c IL-12 diffusion through collagen after 4 h was measured by ELISA. Mean ± SD of three experiments performed in duplicates. P = 0.032; Kruskal–Wallis test with Dunn’s Multiple Comparison Correction Test