Fig. 6.

Spreading of IFNγ signaling in tumor xenografts treated in vivo with collagenase D. a CXCL9 fold induction along the tumor sections in mice treated with IFNγ alone (50 ng per tumor) or together with collagenase D (2.5 μg per tumor). Mean ± SEM of six independent tumors for each treatment. For CXCL9 induction in individual tumors see Supplementary Fig. 6b. ***P = 0.0006; Paired t-test, t = 4.035 and degree of freedom = 20. The red dotted line marks the threshold of 50 CXCL9 mRNA copies per section of the tumor. Each section represents 0.66 mm thick, contains 30 tumor slices, and about 1–4 millions cells. The drawing below shows the diffusion of CXCL9 gradient along the tumor taking into account the threshold. b Quantification of CXCL9 staining area in immunohistochemistry images of several sections of tumors treated either with IFNγ alone or combined with collagenase D. Representative images are shown in Supplementary Fig. 8