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. 2017 Oct 6;7:12751. doi: 10.1038/s41598-017-13095-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow Cytometric Analysis for Tumor Infiltrating Lymphocytes 5 Days After aFP Treatment in the One Tumor Mouse Model. Flow cytometry analysis was performed to confirm whether CD3+ and CD8+ lymphocyte, and regulatory T cell (Treg) numbers were affected by aFP. (a) Proportion of CD3+, CD8+ and CD4+ T cells and Treg normalized to tumor weight. (b) Ratio of CD8+ T cells to Tregs (CD4+ Foxp3+). (c) Proportion of antigen specific CD8+ T cells normalized to tumor weight and the percentage of antigen specific CD8+ T cells normalized to total number of CD8+ T cells. The bars represent SD. * P < 0.05, **P < 0.005.