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. 2017 Oct 6;7:12751. doi: 10.1038/s41598-017-13095-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tumor Survival Curves after aFP Treatment with Anti-CD8 Depletion Antibody. To investigate whether adaptive immunity is necessary to eradicate cancer cells after aFP, tumor inoculation was performed with anti-CD8 depletion antibody to ablate CD8+ T cells in the mouse in the one tumor model. Anti-CD8 depletion antibodies were administered intraperitoneally at a dose of 200 µg per mouse every 3 days from one day before tumor inoculation to removal of mice as endpoint. The graph shows Kaplan-Meier survival curves of mice receiving tumor inoculation and anti-CD8 depletion antibody. The significance values for the difference between the survival curves are: anti-CD8+ aFP vs. aFP: P < 0.005.