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. 2017 Apr 17;38(10):1412–1424. doi: 10.1038/aps.2017.35

Figure 6.

Figure 6

The C-terminal polybasic regions of γ-secretase substrates are important for cleavage. (A) Sequence alignment of a subset of γ-secretase substrates (human C99 and human Notch family). C-terminal polybasic regions are marked by the red outline. (B) Mutational analysis of the positively charged conserved membrane junction sites (K53 of C99 or R1758 of Notch1) and the polybasic regions (K54-K55 of C99 and K1759-R1762 of Notch1) C-terminal to the junction site. (C) Schematic diagram of a PIP2-binding model of the substrate C-terminal polybasic regions. Error bars=SEM, n=3, P-values (two-tailed Student's t-test versus WT): *P<0.05; **P<0.01, ***P<0.001.