Fig. 2.—

Timeline showing the use of different genetic data types for molecular-clock analyses. The development and analysis of different data types is illustrated by a range of studies over the past five decades: amino acid sequences (Zuckerkandl and Pauling 1962), microcomplement fixation (Sarich and Wilson 1967b), protein electrophoresis (Nei 1971), nucleotide sequences (Miyata and Yasunaga 1980; Cohn et al. 1984), DNA–DNA hybridization (Wayne et al. 1991), microsatellites (Goldstein et al. 1995), randomly amplified polymorphic DNA (Espinasa and Borowsky 1998), genome complexity (Sharov 2006), amplified fragment length polymorphisms (Kropf et al. 2009), protein folds (Wang et al. 2011), and recombination events (Moorjani et al. 2016).