Table 1.

Distribution of sequences in G4RNA database

	Locations		G4 validation a
			Confirmed G4s	Denied G4s	Inconclusive results
Wild-type sequences	5′UTR	99	79	17	3
	3′UTR	45	41	0	4
	Exonic coding	12	10	2	0
	Intronic	8	8	0	0
	TERRA	1	1	0	0
	Total	165	139	19	7
All sequences	5′UTR	218	108	106	4
	3′UTR	72	45	23	4
	Exonic	21	17	4	0
	Intronic	12	9	2	1
	Artificial	10	5	5	0
	TERRA	1	1	0	0
	Total	334	185	140	9
Techniques
Discrete tests	Probing and SHAPE	167	87	80	0
	Circular dichroism	136	92	44	0
	Expression assay	115	58	54	3
	Melting temperature	104	69	35	0
	NMR	20	19	1	0
	Native gel mobility	16	14	2	0
	Other	20	13	7	0
	Total	578	352	223	3

^a G4 validation presents the outcome of experimental tests in three columns: Confirmed G4s / denied G4s / inconclusive results

UTR, Untranslated Region; TERRA, Telomeric Repeat-containing RNA; SHAPE, Selective 2'-Hydroxyl Acylation analyzed by Primer Extension; NMR, Nuclear Magnetic Resonance.