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. Author manuscript; available in PMC: 2018 Oct 13.
Published in final edited form as: J Mol Biol. 2017 Jul 17;429(20):2975–2995. doi: 10.1016/j.jmb.2017.06.024

Fig. 9.

Fig. 9

JUN/FOS heterodimers bind to multiple sites on MED25. (a) Representative sensorgrams of binding assays between MED25 ACID and 500 nM JUN (red), 500 nM FOS (blue), or 250 nM JUN/FOS (purple). (b) Representative sensorgrams of binding assays between MED25 ACID wildtype (WT) and point mutants as labeled, and 500 nM FOS. (c) Representative sensorgrams of binding assays between MED25 ACID wildtype Association Dissociation (WT) and point mutants as labeled, and 250 nM JUN/FOS. Note that sensorgrams corresponding to Tyr487Ser and Arg466Glu are shown as dotted and dashed lines, respectively, to aid in visualization because they are almost completely overlapping. See Fig. S10 and Table S6 for further quantification of JUN/FOS-MED25 and FOS-MED25 interactions.