Table 2.

Inhibition of the hydrolysis of model substrates by β-galactosidases from bovine liver (5 mM HEPES buffer, pH 7.50) and A. oryzae (50 mM acetate buffer, pH 5.00) in the presence of galactonoamidines 1d, 1u–y; R = -CH₂-C₆H₄-CH₃

Compound	Glycon structure	β-galactosidase (Bovine Liver)		β-galactosidase (A. oryzae)
		Ki ± ΔKi [nM]	IC50 [nM]	Ki ± ΔKi [nM]	IC50 [nM]
Galactonoamidines with modifications in the glycon
1d		0.07 ± 0.01	57.6	8.0 ± 0.5	36
1u		0.60 ± 0.07	850	2,400 ± 300	33,300
1v		1,500 ± 220	10,800	3,400 ± 300	41,400
1w		840 ± 60	9,200	1,400 ± 200	40,700
Control compounds
1x		2,000 ± 200	> 50,000	150 ± 20	830
1y		2,700 ± 260	8,700	615 ± 30	> 50,000