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. Author manuscript; available in PMC: 2018 Oct 15.
Published in final edited form as: Bioorg Med Chem. 2017 Sep 15;25(20):5790–5798. doi: 10.1016/j.bmc.2017.09.013

Fig. 2. Phage display of functional 2G12.

Fig. 2

(A) Coding regions of pHP153-2G12z and pHP153-2G12h. In both cases, the light chain and heavy chain VH-CH fragment were coexpressed in the periplasm using an stII signal sequence. A FLAG epitope was included at the light chain C-terminus. (B and C) Phage ELISA of particles produced from pHP153-2G12z (B) or pHP153-2G12h (C). The anti-FLAG antibody M2 was used to confirm expression of the construct.