Fig. 5. Characterization of individual clones from limited diversity libraries.

(A) Sequences of mutants from Lib1 and Lib2 that were further studied. The WT residue identity at each position is indicated, those positions that were identical to WT are shaded. (B) ELISA of phage displayed 2G12 and mutants against gp120, M2 (anti-FLAG), and BSA. The WT 2G12 phage were tested at a phage titer of ~10¹⁰ iu/mL whereas the variants were tested at ~10¹¹–10¹² iu/mL. (C) ELISA of purified 2G12 mAb, and variants L1–42 and L1–83 against gp120 and BSA. The EC50s for gp120 were 2.3 pM for 2G12, and 1.0 and 1.6 µM for L1–42 and L2–83, respectively.